This project involves the collection of additional preliminary data for revision of the R01 submission of the same name. Preliminary data collection will focus on development and implementation on a small group of participants, strategies to measure and provide a thorough analysis of participants’ physical activity and caloric and nutritional intake/restriction relative to the tissue analysis. Additionally, intestinal tissue will be collected and Single Cell RNA-Sequencing will be performed and compared with bulk gene expression that has already been performed on intestinal tissue of these participants to demonstrate the feasibility and potential implications for the parent project.